As research on the nutrition of feed- and food-producing insects is growing exponentially, it must be acknowledged that today’s research is not limited in quantity. However, the knowledge generated in research labs is still highly variable and difficult to compare. To some extent, current research may fail in reproducibility because of unstandardized terminology, variable and poorly described experimental setups, improper statistical considerations and analytical methods, and the overall lack of harmonised methods. Working Group 5 will address current challenges and highlight opportunities in insect nutrition research through contributions to the improvement of experimental designs and setups, exploration of (new) tools for nutrient analysis, evaluation and studies on insect metabolism, in close collaboration with WGs 1-4.