The protein and amino acid profiles of feedstocks greatly affect animal development, growth and microbiota. Moreover, some amino acids are essential, while their provision is dependent on the feedstocks used for insect farming. Working group 2 will identify what is known on protein and amino acid requirements for the aforementioned edible insect species, and draft scientific protocols for determining unknown amino acid requirements thereby providing input for WG5. Moreover, WG2 will particularly collaborate with WG1 to better understand protein availability from different feedstocks as found in by-products used as feed.